Attuale ruolo Ricercatore confermato
SSD BIO10
Edificio F
Piano 1
Studio F171
Telefono +39 06 7259 6471
Mail massimo.bottini@uniroma2.it
Biographical
Dr. Massimo Bottini received a Ph.D at the University of Roma Tor Vergata in Sensorial and/or Learning systems, Rome, Italy. In 2002, Dr. Bottini contributed to develop new carbon- and silica-based particles for biomedical applications for approximately 7 months period at the Sanford Burnham Medical Research Institute (SBMRI) and the University of California San Diego (UCSD), in La Jolla, CA. From 2004 to 2006, he joined the laboratory of Dr. Tomas Mustelin as a post-doc at the SBMRI and worked on the development of nano-engineered particles for the delivery of drugs to diseased tissues. From 2007 to 2008, he worked in the laboratory of Dr. Robert C. Liddington on the development of new technologies to measure the intra-molecular forces of cytoplasmic proteins by means of force spectroscopy. In 2009, he was recruited as an Assistant Professor at the University of Roma Tor Vergata. In 2012 he was promoted Adjunct Assistant Professor at the SBMRI. He was awarded as a John Vaughan Scholar by the Arthritis National Research Foundation in 2011 and 2012.
Education
B.S. Electronic Engineer
University of Roma Tor Vergata, Rome, Italy, 2000
Ph.D in Sensorial and/or Learning Systems
University of RomaTor Vergata, Rome, Italy, 2004
Post-doctorate
Sanford Burnham Medical Research Institute, La Jolla, CA, 2004-2006
Staff Scientist
University of California San Diego, La Jolla, CA, 2007-2008
Academic positions
Adjunct Investigator
University of California San Diego, La Jolla, CA, 2006
Assistant Professor
University of Rome Tor Vergata, Roma, Italy, 2009
Adjunct Assistant Professor
Sanford Burnham Medical Research Institute, La Jolla, CA, 2012
Teaching
Dr. Bottini teaches 6 credit-course of Nanomedicine, formerly named Cell Structures and Dynamics, at the Faculty of Engineering since 2009. He mentored one Medical Engineering thesis student and several visiting PhD students and post-docs at the SBMRI.
Research
Development of nanoparticles for the transport of drugs for the therapy of osteoarthritis and rheumatoid arthritis; in collaboration with the La Jolla Institute for Allergy and Immunology (La Jolla, USA).
Analysis of the interaction of plasma proteins with the surface of nanoparticles used as drug delivery systems; in collaboration with the Karolinska Institutet and the Sanford Burnham Medical Research Institue (La Jolla, USA).
In vivo enzymatic degradation of nanoparticles used as drug delivery systems; in collaboration with the Karolinska Institutet.
Analysis, by means of atomic force microscopy, of the morphological and mechanical properties of cytoskeletric proteins, cell organelles and plasma membranes of cells relevant for the development of arthritis; in collaboration with the the Sanford Burnham Medical Research Institue (La Jolla, USA) and the La Jolla Institute for Allergy and Immunology (La Jolla, USA).
CENNI BIOGRAFICI
Il Dott. Massimo Bottini ha conseguito il Dottorato in Sistemi Sensoriali e/o di Apprendimento presso l’Universita’ di Roma Tor Vergata. Durante il 2002 il Dott. Bottini ha trascorso circa 7 mesi presso il Sanford Burnham Medical Research Institute (SBMRI) e la University of California San Diego (UCSD) presso La Jolla, CA, ed ha contribuito allo sviluppo di particelle basate su carbonio e silicio per applicazioni biomedicali. Dal 2004 al 2006 e’ stato post-doc presso il laboratorio del Dr. Tomas Mustelin al SBMRI lavorando sullo sviluppo di nanoparticelle ingegnerizzate per il trasporto di farmaci in specifici tessuti. Dal 2007 al 2008 e’ stato staff scientist presso il laboratorio del Dr. Robert C. Liddington sviluppando nuove tecnologie per la misura delle forze intra-molecolari di proteine citoplasmatiche tramite spettroscopia a forza. Dal 2009 e’ Ricercatore presso l’Universita’ di Roma Tor Vergata. Nel 2012 e’ stato promosso Adjunct Assistant Professor presso il SBMRI. E’ stato nominato John Vaughan Scholar dalla Arthritis National Research Foundation nel 2011 e 2012.
FORMAZIONE
Laurea in Ingegneria Elettronica
Universita’ di Roma Tor Vergata, Roma, Italia, 2000
Dottorato in Sistemi Sensoriali e/o di Apprendimento
Universita’ di Roma Tor Vergata, Roma, Italia, 2004
Post-doctorate
Sanford Burnham Medical Research Institute, La Jolla, CA, 2004-2006
Staff Scientist
University of California San Diego, La Jolla, CA, 2007-2008
POSIZIONI ACCADEMICHE
Adjunct Investigator
University of California San Diego, La Jolla, CA, 2006
Ricercatore
Universita’ di Roma Tor Vergata, Roma, Italia, 2009
Adjunct Assistant Professor
Sanford Burnham Medical Research Institute, La Jolla, CA, 2012
ATTIVITA’ DIDATTICA
Dal 2009 il Dott. Bottini e’ titolare del modulo 6 CFU di Nanomedicina (precedentemente chiamato Strutture e Dinamiche Cellulari) presso la Facolta’ di Ingegneria. E’ stato relatore di n.1 tesi di laurea magistrale in Ingegneria Medica e svolge attivita’ di tutoraggio di visiting PhD students e post-docs presso il SBMRI.
ATTIVITA’ SCIENTIFICA
Sviluppo di nanoparticelle per il trasporto di farmaci per la terapia dell’osteoartrite e dell’artrite reumatoide; in collaborazione con il La Jolla Institute for Allergy and Immunology (La Jolla, USA).
Analisi dell’interazione delle proteine plasmatiche con la superficie di nanoparticelle usate come trasportatori di farmaci; in collaborazione con il Karolinska Institutet ed il Sanford Burnham Medical Research Institue (La Jolla, USA).
Degradazione enzimatica in vivo di nanoparticelle usate come trasportatori di farmaci; in collaborazione con il Karolinska Institutet.
Analisi, mediante uso della microscopia a forza atomica, delle proprieta’ morfologiche e meccaniche di proteine del citoscheletro, di organelli cellulari e della membrana plasmatica di cellule associate a patologie artritiche; in collaborazione con il Sanford Burnham Medical Research Institue (La Jolla, USA) ed il La Jolla Institute for Allergy and Immunology (La Jolla, USA).
PUBBLICAZIONI ULTIMI 5 ANNI
1. Sacchetti C., Liu-Bryan R., Magrini A., Rosato N., Bottini N., Bottini M. Polyethylene-glycol-modified single-walled carbon nanotubes for intra-articular delivery to chondrocytes. ACS Nano. 2014; 8 (12): 12280-12291.
2. Bhattacharya K., Sacchetti C., El-Sayed R., Fornara A., Kotchey G.P., Gaugler J.A., Star A., Bottini M., Fadeel B. Enzymatic ‘stripping’ and degradation of PEGylated carbon nanotubes. Nanoscale. 2014; 6 (24): 14686-14690.
3. Stanford S.M., Aleman Muench G.R., Bartok B., Sacchetti C., Kiosses W.B., Sharma J., Maestre M.F., Bottini M., Mustelin T., Boyle D.L., Firestein G.S., Bottini N. TGFβ responsive tyrosine phosphatase promotes rheumatoid synovial fibroblast invasiveness. Annals of the Rheumatic Diseases. 2014 [Epub ahead of print]
4. Kuzynski M., Goss M., Bottini M., Yadav M.C., Mobley C., Winters T., Poliard A., Kellermann O., Lee B., Millan J.L., Napierala D. Dual role of the Trps1 transcription factor in dentin mineralization. J Biol Chem. 2014; 289 (40): 27481-27493.
5. Bottini M., Sacchetti C., Pietroiusti A., Bellucci S., Magrini A., Rosato N., Bottini N. Targeted nanodrugs for cancer therapy: prospects and challenges. J Nanosci Nanotechnol. 2014 Jan; 14 (1): 98-114.
6. Bottini M., Sacchetti C., Rapini N., Rosato N., Magrini A., Bottini N. Nanosystem for targeting Treg in vivo. Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. 3: 300-303.
7. Campagnolo L., Massimiani M., Palmieri G., Bernardini R., Sacchetti C., Bergamaschi A., Vecchione L., Magrini A., Bottini M., Pietroiusti A. Biodistribution and toxicity of single wall carbon nanotubes in pregnant mice. Particle and Fibre Toxicology, 2013; 10 (1): 21.
8. Sacchetti C., Rapini N., Magrini A., Cirelli E., Bellucci S., Mattei M., Rosato N., Bottini N., Bottini M. In vivo targeting of intra-tumor regulatory T cells using PEG-modified single walled carbon nanotubes. Bioconjugate Chemistry, 2013; 24 (6): 852-858.
9. Sacchetti C., Motamedchaboki K., Magrini A., Palmieri G., Mattei M., Bernardini S., Rosato N., Bottini N., Bottini M. Surface PEG conformation influences the protein corona of PEG-modified single-walled carbon nanotubes: potential implications on biological performance. ACS Nano, 2013; 7 (3):1974-1989.
10. Bottini M., Bottini N. Modified carbon nanotubes: from nanomedicine to nanotoxicology. SPIE Conference Proceedings 2012. 2012; 8462: 84620T.
11. Bottini M., Rosato N., Gloria F., Adanti S., Corradino N., Bergamaschi A., Magrini A. The public optimism towards nanomedicine. International Journal of Nanomedicine, 2011; 6: 3473-3485.
12. Bottini M., Rosato N., Bottini N. PEG-modified carbon nanotubes in biomedicine: current status and challenges ahead. Biomacromolecules. 2011; 12 (10): 3381-3393.